Spatial variability of chlorine in tobacco planting soils was invesitigated to provide theoretical basis for precision management of soil nutrients in hilly areas in Huili, Sichuan Province using geostatistical methods and GIS technique with two sampling scales. In the tobacco plantation at the small scale, the results analyzed by semivariograms indicated that chlorine content was moderately spatially dependent, the range of spatially dependent was 828.5 m, and both structural factors and the random factors equally affected the spatial variability of chlorine content. The results analyzed by anistropic semivariogram indicated that chlorine content had a stronger anisotropic structure in the aspect of NE45°. According to the Ordinary Kriging Method, the equivalence of chlorine content was distributed from northeast to southwest, and the chlorine content was negatively related to slop and elevation of the tobacco plantation. At the micro scale, the results analyzed by semivariograms indicated that the chlorine content showed the pure nugget effect, the spatial variability of chlorine content was fundamentally influenced by the random factors.