In order to provide on site chemical analysis data of tobacco leaves to quality agent at purchase, NIRS method was used to determine the chemical component of tobacco leaves. The ten points of handed tobacco leaf surface was combined into one mean spectrum, which was correlated with the determination results of chemical components by flow analyzer, and the mathematical models were established with average correlation coefficients over 0.9. The designed NIRS model is reliable and practical for chemical components determination of handed tobacco leaves.