Chlorine content, distribution and accumulation, yield and quality of flue-cured tobacco were influenced remarkably by different amount of chlorine applied. Chlorine content and accumulation increased remarkably as increasing chlorine rate. There was a linear relationship between chlorine rate and chlorine content or accumulation. Chlorine distribution in upper leaves increased evidently as increasing the amount of applied chlorine from 0 kg/hm2 to 48 kg/hm2; and distribution in middle and lower leaves increased remarkably as increasing the amount of applied chlorine to 96 kg/hm2; chlorine distribution in stem and roots had degressive trend as decreasing the amount of applied chlorine. Yield, quality and chemical composition were the best with 24 kg/hm2 of chlorine applied; no chlorine applied or applied chlorine more than 24 kg/hm2 had worse effects on yield and quality.