The bacteriostatic tests of total 12 pesticides against tobacco wildfire disease (Pseudomonas syringae pv. tabaci）were investigated by cup-plate method in laboratory. Results showed that high inhibitory effect occurred in the treatment with 72% agricultural streptomycin, 50% thiram, 25% bromothalonil, and 10% methylene dithiocyanate. The effect of various treatment combinations was further investigated to find the optimum pesticide. The treatment combination exhibited higher inhibitory effect. The better combination included agricultural streptomycin + thiram, thiram + methylene dithiocyanate, agricultural streptomycin + Copper nitrosulfuric oxytetracycline hydrochloride+ alkali copper sulfate, + methylene dithiocyanate alkali copper sulfate.