Different crosses of red sun-cured tobacco were made and the heterosis of major agronomic and yield traits of 10 F1 were studied. The results showed that the heterosis of leaf number, plant height and intermodal distance were among parents, several crosses had heterosis over parent. Leaf length, leaf width and stem girth had significant heterosis over parent. Most of leaf number and plant height had negative heterosis. Positive and negative heterosis of intermodal distance was each 50%. Most of leaf length had positive heterosis. All leaf width and stalk circumference had positive heterosis. The results of correlation and regression analysis showed that leaf width and stalk circumference were significantly correlated with the average parents. Plant height had no significant correlation with the average parents. Yields had strong heterosis over parent.