By collecting, collating the sun-cured tobacco germplasm in Heilongjiang Province, we collected a total of 348 cataloging of sun-cured tobacco germplasm, conducted the research on agronomic traits and biological traits of each species, and selected a number of good agronomic traits of the species widely used in production and breeding in Heilongjiang Province, which provided a guiding role for the development of sun-cured tobacco in Heilongjiang Province.