Artificial inoculation in tobacco field was conducted to identify the resistance of 69 tobacco germplasm to PVY. 11 germplasm exhibit high resistance to PVY, they are C151, Havana 10, Balin-Shaiyan and Criollo salteno 11, among which there are 3 flue-cured tobacco varieties, 3 sun-cured tobacco varieties, 3 Turkish tobacco varieties and 2 cigar tobacco varieties, respectively. C151 is a flue-cured tobacco variety with white flowers, and 22 to 24 long-ellipse leaves available in field, the leaf color is green, so C151 can be a useful breeding parent.