In order to well understand and utilize the conserved tobacco germplasm resources, the main characters of 86 flue-cured tobacco germplasms were studied in 2007. The results showed that the main characteristic difference occurred in plant height, stem, leaf number, pitch, leaf length and leaf width, ranging in 110.4-326.2 cm, 6.3-12.9 cm, 2.5-8.8 cm, 15.2-59.0 leaves, 42.3-77.2 cm, 16.0-38.0 cm, respectively, and the coefficients of variation were 13.5 %, 9.4%, 21.2%, 17.1%, 10.3%, 13.6%, respectively. The Shannon index reached 2.090, 2.133, 2.167, 1.916, 2.172 and 2.189, respectively. It is concluded that the resources in the main characters of flue-cured tobacco have high level genetic diversity, and the main characters of most resources are available for breeders.