The field experiment were conducted to study the effect of combining application of Bio-organic fertilizer and chemical fertilizer on the N, P and K absorption of flue-cured tobacco. The results showed that,，it is improved the accumulation of N, P, K in the root, stem and leaf of flue-cured tobacco. The order of accumulation of N was T3>T2>T1>ck, it was 5.29g,、4.84g,、4.20g、3.55g. The order of accumulation of K was T2>T1>T3>ck. It was 4.92ｇ, 4.59ｇ, 4.58ｇ, 3.92ｇ. The absorption velocity of N was greatly improved before 50 days after transplanted. The effect of applied 50 percent organic N of bio-organic fertilizer on the absorption velocity of P was improved than contrast before 50 days, declined by applied 75% organic N utilized the bio-organic fertilizer, it was improved especially in leaf. It was obviously heightened by applied the bio-organic on 50 days after transplant, and it was high a little at other time. It is also increased the contents of N, P, K of the root, stem and improved the contents of flue-leaf.