Cluster analysis was employed on the chemical compositions of 97 flue-cured tobacco leaf samples (C3F) from seven counties of Baise, Guangxi to enhance tobacco leaf utilization, tobacco production technology and related cultivation management in different regions. Results showed that three regions could be divided in Baise. The first included Longlin, Xilin and Tianlin, and the second included Leye, Lingyun and several towns of Debao, such as Xingwang, Wuping, and Jingde etc. The third included most parts of Jingxi and some towns of Debao, such as: Puxu, Duan, Lutong, Dongling, Najia, etc. The flue-cured tobacco leaves in the three parts exhibited evident quality characteristics including appearance quality, chemical composition and sensory quality.