According to the introduction procedure of tobacco variety, K326LF was introduced from Brazil to Guizhou by Guizhou Tobacco Corporation in 1998. Introduction test, variety regional test and productive demonstration were conducted in 1998-2003. The results showed that K326LF had the same resistance to major diseases as K326, its cured leaf yield and value were 182.10 kg/ha and 1 252.95 yuan/ha, respectively, which was higher than those of K326. The main chemical components of flue-cured tobacco leaves and smoking quality met the raw material requirement in Chinese-style cigarettes. K326LF is a promising variety in China at present.