不同產(chǎn)區(qū)烤煙中主要生物堿含量和組成比例分析
Analysis on Major Alkaloids Contents and Constituent Proportions of Flue-cured Tobacco from Different Tobacco Production Regions
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摘要: 采用氣相色譜法對(duì)我國(guó)4個(gè)烤煙主產(chǎn)區(qū)（湖南、貴州,、云南,、山東）煙葉的4種主要生物堿（煙堿、降煙堿,、新煙堿,、新煙草堿）含量進(jìn)行了分析測(cè)定。結(jié)果顯示,，該測(cè)定方法的RSD均小于3.5%,，各生物堿的平均回收率在98.6%～103.0%之間，說明該法測(cè)定結(jié)果準(zhǔn)確,、可靠,；4種主要生物堿含量水平排序?yàn)椋簾焿A＞降煙堿＞新煙草堿＞新煙堿；4個(gè)產(chǎn)煙區(qū)總生物堿平均含量排序?yàn)楹希举F州＞云南＞山東,；產(chǎn)區(qū)煙葉中總生物堿含量與栽培品種關(guān)系密切,。對(duì)檢測(cè)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)分析，結(jié)果發(fā)現(xiàn)4個(gè)產(chǎn)區(qū)煙葉在主要生物堿及總生物堿含量上存在極顯著差異（降煙堿除外）,，新煙堿,、新煙草堿這2種主要生物堿占總生物堿的比例也存在極顯著差異。Abstract: By using gas chromatography, the authors measured and analyzed the contents of four major alkaloids, including nicotine, nornicotine, anabasine, anatabine in flue-cured tobacco from Hunan, Guizhou, Yunnan and Shandong Province. Results showed that the relative standard deviations were below 3.5% and the average recovery of alkaloids were 98.6% - 103.0%. The content of alkaloids from high to low was nicotine＞nornicotine＞anatabine＞anabasine, and the average level of total alkaloid in four production regions was in the sequence: Hunan＞Guizhou＞Yunnan＞Shandong, which was also closely related with cultivars. Based on the statistical analysis of data, leaves from the four major production regions were significantly different in major alkaloids and total alkaloid at 0.01 level (except for nornictine), which also held true in the proportions of anabasine and anatabine in total alkaloid.
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