普通煙草WDR 基因的克隆與序列分析
Cloning and Sequence Analysis of a WDR Gene from Common Tobacco
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摘要: 在煙草腺毛的發(fā)育中,，WD40-bHLH-MYB 復(fù)合體起著重要的調(diào)控作用。利用RT-PCR 從云煙85 中克隆得到一條普通煙草（Nicotiana tobacum）WDR（NtWDR）全長cDNA 序列,，GeneBank 登錄號為GQ260131,。NtWDR 基因cDNA 全長1 433 bp，具有一個1 029 bp 的開放閱讀框（ORF）,、一個147 bp 的5′非翻譯區(qū)和一個257 bp 的3′非翻譯區(qū),。該基因編碼一個342 aa 的蛋白質(zhì)，預(yù)測的分子量為38.49 kDa,，等電點為4.81,。BLASTs 和NCBI 保守域收索表明，NtWDR 是AN11/TTG1型WD40 蛋白,。系統(tǒng)進化和結(jié)構(gòu)特征分析表明,，NtWDR 基因是PhAN11 和Le113964R 基因的垂直同源基因。Abstract: MYB-bHLH-WD40 protein complex plays a crucial role in regulating the trichome differentiation in the tobacco (Nicotiana tabacum). By using RT-PCR method, a WDR (NtWDR) gene was isolated from common tobacco Yuyan 85 and the gene accession number is GQ260131. NtWDR cDNA is 1 433 bp in length with a 1 029 bp open reading frame (ORF) as well as a 147 bp 5′-untranslated region (UTR) and a 257 bp 3′-UTR. The encoded NtWDR protein is 342 aa with a calculated molecular weight (Mw) of 38.49 kDa and a isoelectric point (pI) value of 4.81. BLASTs and NCBI conserved domain search indicated that NtWDR was AN11/TTG1-type WD40 proteins. Phylogenetics analysis and structural characterizations identified NtWDR to be orthologs of PhAN11 and Le113964R.
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