煙草鉀離子通道基因NKT5 的克隆和序列分析

Cloning and Sequence Analysis of Potassium Ion Channel Gene NKT5 in Nicotiana Tabacum

摘要: 本研究運(yùn)用已經(jīng)獲得的1 條490 bp 的普通煙草鉀離子（K+）通道基因的中間片段來(lái)設(shè)計(jì)特異性引物，應(yīng)用RACE方法獲得其3'和5'末端cDNA 序列。通過(guò)三段cDNA 序列拼接結(jié)合全長(zhǎng)克隆測(cè)序驗(yàn)證,，獲得一個(gè)未報(bào)道的普通煙草K+通道基因,，并將其命名為NKT5（NCBI 登錄號(hào)為HM010922）。NKT5 的cDNA 全長(zhǎng)為2 365 bp,，其中5'端非編碼區(qū)190 bp,、3'端非編碼區(qū)249 bp、編碼區(qū)1 926 bp,，編碼641 AA,。對(duì)該基因進(jìn)行了序列分析及功能預(yù)測(cè)，為進(jìn)一步闡明其在煙草吸鉀及抗逆過(guò)程中的功能及分子機(jī)制奠定了基礎(chǔ),。

Abstract: Based on a 490 bp middle segment of K ion channel gene, a pair of specific primers was designed to acquire 5' and 3'-ends of cDNA by RACE strategy. An unreported K ion channel gene in tobacco (Nicotiana tabacum) named as NKT5 was obtained by splicing three cDNA sequences and sequencing full-length cloning. The full-length cDNA of NKT5 is 2 365 bp long with 190 bp 5'-untranslated region (UTR), 249 bp 3'-UTR and 1 926 bp coding sequence. The deduced amino acid is 641 AA long. The gene sequence analysis and function prediction were carried out in order to clarify molecular mechanisms of potassium absorption and stress-resistance in the tobacco.