烘烤過(guò)程中烤煙細(xì)胞壁生理變化研究

Cell Wall Physiological Changes of Tobacco Leaves during Curing Process

摘要: 采用電熱式溫濕自控密集烤煙箱對(duì)烤煙上部煙葉在烘烤中細(xì)胞壁水解酶和主要組分隨溫濕度的變化進(jìn)行了研究,。結(jié)果表明：果膠甲酯酶(PME),、多聚半乳糖醛酸酶(PG)和纖維素酶在38～48 ℃酶活性較高。相關(guān)性分析表明：PME 與纖維素酶呈極顯著正相關(guān),。烘烤中可溶性果膠含量不斷增加,，原果膠、總果膠和纖維素含量不斷降低,，其中果膠(總果膠,、原果膠及可溶性果膠)與PG 的關(guān)系比果膠與PME 的關(guān)系更密切。

Abstract: The change pattern of major components and hydrolase in the cell wall of upper tobacco leaves with temperature and humidity during the bulk flue-curing process was studied by using the electric-heated flue-curing barn designed by Henan Agricultural University. The results showed that pectin methylesterase (PME), polygalacturonase (PG) and cellulase were highly active at 38 to 48 oC. Correlation between PME and cellulase was highly positive significant. During curing, leaf soluble pectin content increased continuously while protopectin, total pectin and cellulose contents decreased continuously. Correlation analysis showed that the relationship of pectin (total pectin, protopectin and soluble pectin) with PG was more closely than that with the PME.