斜紋夜蛾核多角體病毒郴州株的形態(tài)學,、基因組酶切圖譜及生物活性測定

斜紋夜蛾核多角體病毒郴州株的形態(tài)學、基因組酶切圖譜及生物活性測定

摘要: 對斜紋夜蛾核多角體病毒(SpliNPV)郴州株的多角體形態(tài)進行了掃描電鏡觀察,，多角體直徑在1.5～2.5 μm 之間,；多角體經(jīng)切片后透射電鏡觀察，每個病毒囊膜內(nèi)包被的核衣殼數(shù)為2～7 個,；提取SpliNPV 郴州株和廣州株的基因組進行限制性內(nèi)切酶分析,，兩者的圖譜比較近似；分別以不同濃度的兩株SpliNPV 病毒懸液感染3 齡初斜紋夜蛾幼蟲,，其半致死濃度分別為1.8×106 OBs/mL 和4.2×106 OBs/mL,，兩者在統(tǒng)計學上沒有顯著差異。

Abstract: A strain of Spodoptera litura nucleopolyhedrovirus (SpliNPV) isolated in Chenzhou was investigated on morphology, genomic restriction endonuclease analysis and biological activity assay. By using a scanning electron microscope, its polyhedra were found between 1.5-2.5 μm in diameter. By using TEM, it was found each virion particle included 2-7 nucleocapsids. SpliNPV Chenzhou strain genome was analyzed by restriction endonuclease and compared with a Guangzhou strain, they had a similar profile. When assayed on 3r instar of Spodoptera litura larvae, the half-lethal concentration of SpliNPV Chenzhou strain and Guangzhou strain were 1.8×106 OBs/mL and 4.2×106 OBs/mL, respectively, they were not significantly different each other.