枯草芽孢桿菌SH7 抑菌蛋白產(chǎn)生的最佳發(fā)酵條件及溫室防效
Optimal Fermentative Condition of Antagonistic Protein Produced by Antagonistic SH7 and its Control Effects in Greenhouse
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摘要: 枯草芽孢桿菌SH7可抑制煙草青枯病菌的生長(zhǎng),，利用硫酸銨沉淀法從發(fā)酵液中提取抑菌蛋白質(zhì)，測(cè)定其對(duì)煙草青枯病菌抑菌活性,，從而確定抑菌蛋白產(chǎn)生的最佳培養(yǎng)基和培養(yǎng)條件,。本實(shí)驗(yàn)產(chǎn)生抗菌蛋白的最佳培養(yǎng)基為NA液體培養(yǎng)基，最佳發(fā)酵條件為：pH 6.0,，26 ℃,，裝液量100 mL，170 r/min振蕩培養(yǎng)96 h,。粗抑菌蛋白在pH為9.0和10.0時(shí),，其抑菌活性仍保持為86%, 88%，對(duì)堿穩(wěn)定,；在100 ℃和121 ℃處理20 min,，其抑菌活性仍保持為83.1%, 68.0%，具有良好的熱穩(wěn)定性。在溫室內(nèi),，抗菌蛋白粗提液對(duì)煙草青枯病可取得70.8%的防效,。Abstract: Bacillus subtilis SH7 can inhibit the growth of tobacco Ralstonia solanacearum. In order to investigate the best medium and optimal fermentative condition, antagonistic protein was extracted from fermentative optimal medium by using ammonium sulphate precipitation method, and its antagonistic ability to tobacco Ralstonia solanacearum was measured. Results showed that optimal medium for SH7 was NA medium, and its optimal fermentative condition was pH 6.0, 26 ℃, 100 mL/250mL, 170 r/min, 96 h shaking culture. The antagonistic protein was insensitive to alkaline, and at pH 9.0 or 10.0, its antagonistic ability was 86% and 88%, respectively. At 100 and 121 ℃, 20 min, antagonistic ability was 83.1% and 68.0%, respectively. The control effect of the crude protein to tobacco bacterial wilt in greenhouse was 70.8% by injection.
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