香料煙腺毛的形態(tài)發(fā)生和分泌活動研究
Morphological Development and Secretion Activity of Glandular Trichomes in Oriental Tobacco
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摘要: 為進一步探討香料煙葉腺毛的形態(tài)發(fā)生和分泌活動規(guī)律，為優(yōu)質(zhì)香料煙的開發(fā)提供理論依據(jù),，試驗通過石蠟切片法觀察了腺毛的形態(tài)發(fā)生過程,，利用光學(xué)顯微鏡和掃描電鏡觀察了腺毛的分泌活動。結(jié)果發(fā)現(xiàn),，長柄腺毛和短柄腺毛最早均產(chǎn)生于莖尖高約100～200 μm 的第1 對葉原基上,，腺毛原初細(xì)胞大部分發(fā)育成長柄腺毛；長柄腺毛和短柄腺毛由原表皮細(xì)胞突起分化產(chǎn)生,，經(jīng)過不同的形態(tài)發(fā)生過程逐漸形成,。長柄腺毛一般在煙葉欠熟時分泌活動開始增強，至葉片適熟期進入分泌高峰,，腺頭細(xì)胞外出現(xiàn)大量膠狀分泌物,，但部分腺毛分泌活動滯后,，在煙葉調(diào)制時進入分泌高峰,；短柄腺毛從葉片生長成熟到調(diào)制陳化均沒有觀察到腺頭細(xì)胞外有分泌物,。Abstract: The morphology development and secretion activity of glandular trichomes in oriental tobacco were investigated by paraffin section, optical microscope and scanning electron microscope, respectively. The results showed that both primary long and short trichomes originated from the first and second leaf primordium at 100-200μm, while the initial trichome cells generally developed into long trichomes. The differentiation cells bulging from protoderm developed into long or short trichomes through different morphogenesis evolved. The secretion of long trichomes grandually strengthened in the ver-matured leaves, and came to climax with lots of gummy exudate overflowed from gland cells when the leaves were proper mature. However, the secretion of some trichomes delayed, entering the climax during curing of the leaves. The gummy exudate was hardly observed in the short trichomes during the growth, mature, curing and aging of the leaves.
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