部分烤煙種質(zhì)遺傳多樣性與親緣關(guān)系的RAPD 分析
Genetic Diversity and Genetic Relationship Analysis of Some Flue-cured Tobacco Germplasms Based on RAPD
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摘要: 以22 份烤煙材料為供試材料,，應(yīng)用RAPD（隨即擴(kuò)增多態(tài)性DNA）分子標(biāo)記技術(shù)研究22 份烤煙材料之間遺傳多樣性和親緣關(guān)系,。結(jié)果表明，第一等級(jí)劃分（D=0.30）可以將22 份材料劃分為三大類群。第二等級(jí)劃分（D=0.23）可以將22 份材料劃分為六個(gè)小類群,，其中,，一個(gè)是由云煙系列與親本是特字401 系選的品種及其雜交后代組成的類群；一個(gè)是由多數(shù)美國來源品種組成的類群,；一個(gè)由9804,、巖煙97 和Coker176 三個(gè)品種組成的類群；一個(gè)由CF965 和K07 兩個(gè)品種組成的類群,；紅花大金元和C2 分別獨(dú)立成類,。說明分子標(biāo)記多態(tài)性基本上反映了品種間的遺傳變異。本結(jié)果為煙草品種改良和雜交種選配,，提高育種效率提供了理論依據(jù),。Abstract: In order to provide new evidence for the study of origin and evolution of flue-cured tobacco, genetic diversity and genetic relationships among 22 varieties of flue-cured tobacco were investigated with the technique of RAPD and Morphological Markers. RAPD Cluster analysis was carried out using software Bio ID++. A dendrogram was constructed by using the RAPD data and the integrated data, respectively. According to the dendrogram the varieties could be clustered into three groups at the level of D=0.30 by the first classification. At the level of D=0.23 by the second classification, the varieties could be clustered into 6 groups（Yunyan series and their genetic parent and Tezi 401 and its family progenies,；Majority varieties from American; 9804, Yanyan 97 and Coker176; CF965 and K07; Hongda; C2）. The results of two types of cluster analysis showed somewhat geographical origin and genealogical origin of the variety characteristics.
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