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    拮抗細(xì)菌活性蛋白誘導(dǎo)煙草對CMV 的抗性研究

    Induced Resistance to CMV in Tobacco Treated with Antagonistic Bacteria Active Protein

    • 摘要: 從煙田耕作層土樣中分離到對CMV 有拮抗作用的細(xì)菌菌株B6，通過離子交換層析和凝膠過濾層析初步確定其活性物質(zhì)是分子質(zhì)量大小為40.6 kD 的蛋白質(zhì),?；钚缘鞍渍T導(dǎo)后，對煙草葉片的病毒含量,、過氧化物酶(POD),、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)的活性變化進(jìn)行研究。結(jié)果表明,，拮抗細(xì)菌活性蛋白誘導(dǎo)后煙草葉片內(nèi)CMV 含量明顯降低,隨著誘導(dǎo)天數(shù)的增加病毒的含量呈由多到少再增多的變化趨勢,，POD, PAL 和PPO 等細(xì)胞防御酶的活性增強(qiáng)。

       

      Abstract: The virus disease is one of the major diseases on crops. The control effects and security of antiviral agent are not as good as people’s demands till now. New antiviral agents are in dire need of researching. In this study, we isolated bacterial strain B6 from healthy and sick tobacco soil. The results presented here demonstrated the active material was protein through using cation exchange chromatography and molecular sieve chromatography. The relative molecular mass of subunit was 40.6 kD. The induced resistance to CMV in tobacco was treated by antiviral protein., and the CMV contents and the activity of PPO, POD, PAL were studied. The results showed that with the time extending of inducing, the content of CMV increased at beginning, and then decreased, at last increased again. Antiviral protein could enhance the activity of cytophylaxis enzyme such as PPO, POD and PAL.

       

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