一株煙草花葉病毒生防細(xì)菌的鑒定及代謝物活性研究
Identification and Antivirus Activity of Bacteria Strain By33 Against Tobacco Mosaic Virus
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摘要: 從煙田土樣中分離到1 株對(duì)TMV 有拮抗作用的細(xì)菌By33,，通過(guò)形態(tài)觀察、生理生化試驗(yàn)及16S rDNA 同源性序列分析,，鑒定該菌為解淀粉芽孢桿菌,。通過(guò)對(duì)By33 代謝產(chǎn)物對(duì)TMV 抑制作用測(cè)定發(fā)現(xiàn)，其代謝物質(zhì)對(duì)溫度敏感,，80℃處理30 min,，喪失大部分活性，遇酸沉淀,，堿性條件下穩(wěn)定,，對(duì)蛋白酶不敏感。Abstract: An antagonistic bacteria strain By33 against TMV was isolated from tobacco soils. Basing on morphological observation, physiological & biochemical test and 16S rDNA sequence analysis, By33 was identified as Bacillus amyloliquefaciens. The active substance lost most active control at 80 ℃, and was unstable in acid condition yet stable in neutral or slight alkaline condition, with resistant to proteolytic enzymes.
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