煙草赤星病菌(Alternaria longipes)產(chǎn)角質(zhì)酶液體發(fā)酵條件的優(yōu)化

Optimal Fermentation Condition of Producing Cutinase by Alternaria longipes

摘要: 以基礎(chǔ)培養(yǎng)基（查彼培養(yǎng)基＋碳酸鈣＋蘋果角質(zhì)）為營養(yǎng)優(yōu)化了長柄鏈格孢(Alternaria longipes)產(chǎn)角質(zhì)酶的環(huán)境和營養(yǎng)條件。結(jié)果表明最佳產(chǎn)酶的環(huán)境條件是：培養(yǎng)時(shí)間8 d,，培養(yǎng)溫度25 ℃,，pH 5.5；通過正交試驗(yàn)確定最佳產(chǎn)酶的營養(yǎng)條件是：蔗糖3％,，干酪素1％,，吐溫-80 0.3%；酶活高達(dá)175.2 U/mL,，是基礎(chǔ)培養(yǎng)基酶活力的11.0 倍,。

Abstract: Alternaria longipes is the causal agent of tobacco brown spot, a world-wild tobacco disease. Based on Czapek medium supplemented with CaCO3 and apple cutin, the liquid fermentation conditions were optimized in order to acquire higher cutinase activity. Optimum culture time, temperature and pH were 8 d, 25℃ and 5.5, respectively; and optimum carbon and nitrogen sources were dextrose and maltose instead of NaNO3. According to this fermentation and orthogonal test, the optimum composition of culture medium included dextrose 3％, casein 1％, Tween-80 0.3%. A 11.0-fold higher cutinase activity was obtained compared to the basal medium and the enzyme activity was enhanced from 15.8 U/mL to 175.2 U/mL.