安徽省煙草病毒種類的血清學(xué)鑒定
Serological Identification of Tobacco Viruses Derived from Anhui Province
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摘要: 2007 年從安徽亳州,、宣城和蕪湖等地的煙草產(chǎn)區(qū)共采集181 個(gè)表現(xiàn)明顯病毒病癥狀的病樣,，采用Tas-ELISA 和間接ELISA 方法共檢測(cè)112 個(gè)病樣，其中分別檢測(cè)宣城黃渡,、宣城金壩,、蕪湖紅楊、蕪湖三元各16 個(gè)病樣以及亳州陳店48個(gè)病樣,。結(jié)果顯示,，安徽宣城32 個(gè)病樣中，TMV,、CMV,、PVY 的陽(yáng)性率分別為91%、3%,、50%,，蕪湖32 個(gè)病樣中，TMV,、CMV,、PVY 的陽(yáng)性率分別為66%、0,、75%,，說(shuō)明宣城、蕪湖煙區(qū)侵染煙草的病毒種類以TMV,、PVY 為主,，CMV 為零星侵染；亳州48 個(gè)病樣中,，TMV,、CMV,、PVY 的陽(yáng)性率分別為100%、48%,、8%,，說(shuō)明亳州煙區(qū)侵染煙草的病毒種類以TMV、CMV 為主,，PVY 侵染發(fā)生較少,。Abstract: One hundred and eighty one tobacco samples with obvious virus symptoms were collected from Bozhou, Xuancheng and Wuhu of Anhui Province, 2007. The 112 samples, including 16 samples from Huangdu Xuancheng, Jinba Xuancheng, Hongyang Wuhu, Sanyuan Wuhu, respectively and 48 samples from Chendian Bozhou, were detected by Tas-ELISA and indirect ELISA methods. The results demonstrated that the positive rates of TMV, CMV and PVY of 32 samples from Xuancheng, Anhui were 91%, 3% and 50%, respectively, while those from Wuhu were 66%, 0 and 75%, respectively. It suggested that the major virus types infecting tobacco from Xuancheng and Wuhu were TMV and PVY, while CMV only occurred sporadically. The positive rates of TMV, CMV and PVY of 48 samples from Bozhou were 100%, 48% and 8%, respectively. It showed that the major virus types infecting tobacco from Xuancheng and Wuhu were TMV and CMV, and PVY infection rarely occurred.
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