蒸汽對煙草漂浮苗干根中煙草花葉病毒的失活效果

The Inactivation Effects of Steam to Tobacco Mosaic Virus in Dry Roots of Tobacco Float Seedling

摘要: 為了評價蒸汽用于煙草漂浮育苗舊苗盤殘留物中煙草花葉病毒（Tobacco mosaic virus, TMV）的消毒效果，采用枯斑寄主和K326接種方法,，測定了蒸汽對煙草漂浮苗花葉病株干根中TMV的失活效果,。結(jié)果表明，96℃蒸汽蒸3 min～60 min,，對病苗干根中TMV失活效果高,，可達99.9%。血清學檢測結(jié)果表明,，蒸汽消毒3 min能有效破壞病根樣品中TMV抗原,。推薦舊苗盤TMV蒸汽消毒方法為：苗盤潤濕后96℃蒸汽保持不少于3 min。但在96℃蒸汽中(玉溪，常壓蒸汽)保持5 min后,，普通聚苯乙烯泡沫苗盤在冷卻過程中變形,。

Abstract: In order to evaluate inactivation effects of steam to Tobacco mosaic virus (TMV) in residue of float tray, The inactivation effects to TMV in dry roots of tobacco float seedling were evaluated by local lesion assay and inoculation to tobacco variety K326. 96℃ steam keep 3 minutes to 60 minutes could highly inactivated TMV in dry roots of tobacco float seedling, which inactivation ratio are 99.9% to 100%. TAS-ELISA and TMV-Imunostrip test show that steam could fully destroy TMV antigen in dry tobacco seedling roots. 96℃ steam keep not less than 3 minutes is recommend to apply in used float tray for TMV disinfection. But foamed polystyrene plastic trays became distortion after treated at 96℃ steam for 5 minutes.