fps 轉(zhuǎn)基因烤煙類胡蘿卜素及其降解產(chǎn)物的研究
Studies on Cartenoids and Aromatic Components of the Transgenic Flue- cured Tobacco Expressing fps Gene
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摘要: 為探索法呢基焦磷酸合酶( fps) 對烤煙中類胡蘿卜素及其降解產(chǎn)物的影響, 利用常規(guī)和GS / MS 方法, 對轉(zhuǎn)fps 基因煙草株系( K- 4,、K- 6、K- 17,、K- 35) 烤后煙葉中類胡蘿卜素含量及萜烯類香氣物質(zhì)含量進(jìn)行了分析, 結(jié)果發(fā)現(xiàn), 烤后煙葉類胡蘿卜素含量有較大差異, 與未轉(zhuǎn)基因?qū)φ障啾绕毡橛兴岣? 其中K- 35 含量最高; 8 種類胡蘿卜素降解產(chǎn)物及茄酮,、新植二烯含量有不同程度的提高, 其中K- 35 香氣降解產(chǎn)物含量整體較高, 表明外源fps 基因在煙草中的表達(dá)對類胡蘿卜素合成具有促進(jìn)作用, 從而有利于煙葉品質(zhì)的提高。Abstract: In order to explore the effect of farnesyl pyrophosphate synthase gene (fps) on the flue- cured tobacco, cartenoids and aromatic components of transgenic tobacco plant lines(K- 4,、K- 6,、K- 17,、K- 35) expressing fps gene of Mentha spicata L. were studied.Cartenoids content and the terpene aromatic components of flue- cured tobacco leaves were determined by GS/MS. Cartenoids content of transgenic tobacco leaves increased greatly compared to that of non- transgenic tobacco plants. The differences also existed among different transgenic lines, and the cartenoids contents of K- 35 lines is the highest . 8 kinds of aromatic components degraded from cartenoids were detected, and the content increased compared to the control. Solanone and neophytadiene were also increased in transgenic lines. This results showed that the expression of foreign fps gene in tobacco could improve the aromatic quality of tobacco leaves by enhancing terpene metabolites.
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