SH7對(duì)煙草青枯病的抑菌,、防病作用及其抑菌物質(zhì)的初步研究
A Preliminary Study on Inhibition Activity and Antimicrobial Substance of Antagonistic Bacteria SH7 to Tobacco Wilt
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摘要: 研究了生防細(xì)菌菌株SH7及其除菌發(fā)酵液對(duì)煙草青枯病菌（Ralstonia solanacearum）的抑菌和防病作用,，并對(duì)其產(chǎn)生的抑菌物質(zhì)進(jìn)行了粗提。結(jié)果顯示,，該菌株對(duì)R. solanacearum有很強(qiáng)的抑菌作用,，且其除菌體發(fā)酵液對(duì)煙草青枯病的防效較好。將SH7無(wú)菌發(fā)酵液,、無(wú)菌發(fā)酵液與青枯病菌混合30 min施入土壤29 d后,，兩種處理與對(duì)照相比，防效分別達(dá)到65.99%和53.2%,；SH7菌株產(chǎn)生的抑菌物質(zhì)是SH7除菌發(fā)酵液防病的主要機(jī)制,；SH7分泌的抑菌物質(zhì)可以被硫酸銨沉淀，初步認(rèn)為該抑菌物質(zhì)是蛋白類物質(zhì),。Abstract: The inhibition activity of the strain SH7 to tobacco wilt（Ralstonia solanacearum）, isolated from the rhizopheres of tobacco plants, was studied. Disease control with SH7 bacterium-free ferment was carried out. We extracted its antimicrobial substance, too. The results indicated that the strain SH7 was strongly antagonistic to Ralstonia solanacearum. Compared to those of CK in 29d after treatment, the control effects of SH7 bacterium-free ferment, which included two treatments (watering roots with SH7 bacterium-free ferment and with the 30 min mixed suspension of SH7 bacterium-free ferment and Ralstonia solanacearum) were 65.9% and 53.2%, respectively. It implied that the excretion of SH7 is a very important antagonistism. This antimicrobial substance was purified by ammonium sulfate with 70% saturation, and we pre-concluded that it belongs to a kind of protenlike substances.
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