煙草內(nèi)生青枯病拮抗細(xì)菌的篩選和初步鑒定

Selection and Preliminary Identification of Tobacco Antagonistic Endophytic Bacteria against Ralstonia Solanacearum

摘要: 為了獲得防治煙草青枯病新途徑,，從湖南桂陽(yáng)煙草青枯病重病區(qū)采集的健株上，共分離到內(nèi)生菌株160個(gè),。經(jīng)對(duì)峙培養(yǎng)法測(cè)定，32個(gè)菌株對(duì)煙草青枯菌有拮抗作用,，其中H-1,、H-9和H-11有強(qiáng)拮抗作用，抑菌圈分別為3.0,、3.0,、4.5 mm。根據(jù)其形態(tài)特征和生理生化性狀,，初步鑒定內(nèi)生菌H-1為枯草芽孢桿菌,，H-9和H-11為短芽孢桿菌。

Abstract: One hundred and sixty endophytic bacteria strains were isolated from health tobacco plants in Guiyang county of Hunan province in disease severely infested plots. Thirty two, in particular three endophytic bacteria strains showed relatively strong antagonistic actions against Ralstonia solanacearum. Culturing these three bacteria and Ralstonia solanacearum together, the results showed that three endophytic bacteria H-1, H-9, and H-11 had the best antagonistic effects, and bacteriostasis diameters were 3.0, 3.0, and 4.5 mm, respectively. With regard to morphologic, physiological, and biochemical characterics, strain H-1, H-9, and H-11 were identified. The results showed that H-1 was Bacillus subtilis. H-9 and H-11 were Bacillus brevis. We also identified that they were all endophytic bacteria in tobacco.