拮抗TMV細(xì)菌CZ的最佳發(fā)酵條件及其活性物質(zhì)的初步研究
Preliminary Study on Antiviral Substances Produced by Bacteria Strain CZ and its Optimal Fermentative Conditions
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摘要: 獲得了對(duì)TMV有很強(qiáng)的抑制活性的拮抗細(xì)菌菌株CZ,,其發(fā)酵液的抑病毒率可達(dá)96.73%,。對(duì)CZ菌株的搖瓶發(fā)酵條件進(jìn)行了初步研究。結(jié)果表明,,CZ菌株的適宜搖瓶發(fā)酵條件是:NB培養(yǎng)基,、初始pH 8.0、通氣量30 mL/100mL,、發(fā)酵溫度25℃,、發(fā)酵時(shí)間60 h。并初步確定其拮抗物質(zhì)是蛋白類物質(zhì),。Abstract: The authors studied the fermentation conditions of a kind of antiviral bacteria which was filtrated from the seeds of the tobacco. The results indicated that the most appropriate fermentation medium was NB medium and the optimal fermentation parameters were pH 8.0, 25℃, 60 hours. Its anti-material could be protein.