高效液相色譜法同時(shí)測定煙草中吡蟲啉、多菌靈,、甲基硫菌靈的殘留量
Determination of Imidacloprid, Carbendazim and Thiophanate-methyl in Tobacco by High Performance Liquid Chromatography
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摘要: 用固相萃取-高效液相色譜法對煙草中吡蟲啉（imidacloprid）,、多菌靈（carbendazim）、甲基硫菌靈（thiophanate-methyl）3種農(nóng)藥同時(shí)進(jìn)行殘留分析,。用丙酮/水（1:1,，V/V）提取，二氯甲烷萃取，紫外檢測器分析,，檢測波長為270 nm,，流動(dòng)相為甲醇/水（60:40，V/V）,。試驗(yàn)結(jié)果表明,，方法回收率吡蟲啉為91.9%～97.1%，相對標(biāo)準(zhǔn)偏差為3.97%～10.01%,；多菌靈為92.7%～99.9%,，相對標(biāo)準(zhǔn)偏差為3.15%～5.37%；甲基硫菌靈為87.4%～95.3%,，相對標(biāo)準(zhǔn)偏差為3.05%～4.61%,。該方法操作簡便，分離效果好,，準(zhǔn)確度和精密度均達(dá)到定量分析要求,。Abstract: The residues of three pesticides (imidacloprid, carbendazim, thiophanate-methyl) in tobacco leaves were analyzed by using high performance liquid chromatography. The samples were distilled by acetone/water (v/v=1:1), extracted by dichloromethane and analyzed by using UV detector at 270nm. The liquid phase was methanol/ water(60:40). The analytical results showed that the recovery ratios of imidacloprid was 91.9%-97.1% and the relative standard deviations was 3.97%-10.01%, carbendazim was 92.7%-99.9% and the relative standard deviations was 3.15%-5.37%, thiophanate-methyl was 87.4%-95.3% and the relative standard deviations was 3.05%-4.61%.This method showed simple, good separation effect and high accuracy and precision met the demand of quantitative analysis.
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