應(yīng)用SRAP標記研究煙草種質(zhì)資源的遺傳多樣性

Application of SRAP on Analysis of Genetic Diversity in Tobacco Germplasms

摘要: 用15對SRAP引物組合對46個普通栽培煙草品種，包括30個普通煙草栽培種和16個黃花煙草進行了多態(tài)性分析,，共獲得3036條帶,，其中多態(tài)性條帶為636條，每對引物平均提供42個多態(tài)性標記信息,?；赨PMGA方法得到的聚類分析結(jié)果表明46個煙草品種間的遺傳相似系數(shù)為0.67～0.98，聚類分析可將這46個品種劃分為2個大類群,，同種類型的煙草基本聚合在同一種類群中,，表明SRAP標記是適合煙草種質(zhì)資源遺傳多樣性研究的。

Abstract: To investigate the genetic diversity of tobacco germplasms, 46 common cultivated tobacco varieties/lines, including 30 cultivars of Nicotiana tabacum L. and 16 N. rustica L. varieties, were amplified by 15 SRAP (sequence-related amplified polymorphism) primers selected from 160 primers. The results showed that a total of 3036 SRAP bands were obtained with 15 informative primer combinations, of which 636 bands were polymorphic and the average for per-primer was 42 bands. The UPGMA cluster based on SRAP indicated that 46 samples could be clustered into two groups, the same type tobacco varieties could be approximately clustered together, which indicated that SRAP is useful in revealing the genetic diversity among tobacco germplasms.